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KMID : 0355420190430020072
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Journal of Korean Academy of Oral Health
2019 Volume.43 No. 2 p.72 ~ p.77
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Effect of apoptosis on G361 cells by Cimicifuga rhizoma extract
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Choi Byul-Bo-Ra
Kim Gyoo-Cheon
Hong Jin-Woo
Park Sang-Rye
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Abstract
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Objectives: To investigate whether the cytotoxic effect of Cimicifuga rhizoma extract is associated with cell death in the human keratinocyte (HaCaT) and human melanoma cell lines (G361).
Methods: Apoptosis induced by Cimicifuga rhizoma extract was confirmed by water-soluble tetrazolium salts-1 (WST-1) assay, immunocytochemistry, and western blot. Additionally, the release of cytochrome c and apoptosis-inducing factor (AIF) was visualized by confocal laser scanning microscopy.
Results: The results showed that Cimicifuga rhizoma extract significantly reduced the viability of G361 cells with half-maximal inhibitory concentration (IC 50) of 200 μg/ml, and the apoptotic process was found to occur via the activation of caspase-3 and caspase-9 pathways. Besides, the release of cytochrome c and AIF was also detected.
Conclusions: This study suggests that Cimicifuga rhizoma extract causes apoptosis of human melanoma cells through the intrinsic apoptotic pathway.
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KEYWORD
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Apoptosis, Cimicifuga rhizoma, Melanoma
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